Promega Launches Sanger Sequencing Chemistry Compatible With All CE Platforms

In a major development for research labs worldwide, Promega Corporation has launched the ProDye Terminator Sequencing System—a new Sanger sequencing chemistry that can be used on any capillary electrophoresis (CE) platform. This breakthrough system is compatible with various instruments, including Promega’s own benchtop Spectrum Compact CE System, as well as SeqStudio and Applied Biosystems Genetic Analyzers.

More than 40 years after its invention, Sanger sequencing remains the gold standard for DNADNA, or Deoxyribonucleic Acid, is the genetic material found in cells, composed of a double helix structure. It serves as the genetic blueprint for all living organisms. Read Full Definition sequencing. It is especially valuable for applications such as mitochondrial sequencing, de novo sequencing, and confirming results from next-generation sequencing (NGS). Despite the growing adoption of NGS due to its decreasing cost, Sanger sequencing continues to be the most cost-efficient method for sequencing shorter DNA fragments.

“Because the workflows for sequencing already exist, we designed this kit to integrate seamlessly into current protocols,” said Jay Lemke, Senior Research Scientist at Promega Corporation.

The ProDye Terminator Sequencing System employs a proprietary thermostable DNA polymerase and incorporates the same dyes found in Thermo Fisher Scientific’s BigDye Terminator V3.1 Cycle Sequencing Kit. This innovation offers research labs increased flexibility and efficiency by ensuring that the Promega chemistry can be implemented on virtually any CE instrument available.

With this launch, Promega reinforces its commitment to supporting forensic and research communities by providing tools that enhance the accuracyIn scientific and measurement contexts, "accuracy" refers to the degree of proximity or closeness between a measured value and the true or actual value of the measured quantity. Accuracy indicates how well a measurement reflects Read Full Definition and cost-effectiveness of DNA sequencing.