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Simplyforensic > Blog > Crime Scene Investigation > The Hidden Power Of Forensic Light Source In Forensic Science
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The Hidden Power Of Forensic Light Source In Forensic Science

Simplyforensic
Last updated: February 4, 2025 7:31 pm
By Simplyforensic
Published: February 4, 2025
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Around the world, national DNADNA, or Deoxyribonucleic Acid, is the genetic material found in cells, composed of a double helix structure. It serves as the genetic blueprint for all living organisms. More databases continue to expand, with hundreds of thousands of new profiles added each year – each profile telling its own story of evidence detection and analysis. Behind these numbers lies a remarkable tool: Forensic Light Source (FLS) or Alternative Light Source (ALS), which reveals what human eyes cannot see. These specialized instruments work across wavelengths from ultraviolet to infrared, uncovering everything from dried blood spots to microscopic fiber traces.

Contents
Understanding Alternative Light Source TechnologyBasic Principles of Light-Based DetectionTypes of Forensic Light Sources AvailableKey Components and Their FunctionsUV to IR Spectrum in Evidence DetectionUV Light Applications (300-400nm)Visible Light Range Uses (400-700nm)IR Detection Capabilities (700nm+)Materials and Methods for Light Source AnalysisEquipment Setup RequirementsSafety Protocols and Protective GearEvidence Types and Detection MethodsBody Fluid Detection TechniquesFingerprint Enhancement ProceduresDetection & Enhancement StepsTrace Evidence VisualizationHair and Fiber DetectionBruises, Bite Marks, and Pattern WoundsQuestioned Document ExaminationGunshot Residues and Explosive DetectionDetection of Human Bone Fragments, Tire Tracks, Shoe Prints, and DrugsAdvanced Detection TechniquesMulti-Wavelength Scanning MethodsDigital Documentation SystemsReal-Time Analysis ToolsSystem Limitations and ChallengesEnvironmental Interference FactorsMaterial-Specific Detection IssuesConclusionFAQsWhat are the main types of forensic light sources used in crime scene investigations?How does UV light assist in forensic analysis?What role does infrared light play in forensic examinations?What are some limitations of forensic light source technology?Are there any safety concerns when using forensic light sources? Further Readings & Sources

Light Source Detection involves a fascinating interplay between evidence and electromagnetic waves. For example, consider a latent fingerprint, which is invisible under normal conditions. Specific compounds glow or darken distinctively against their background when the proper wavelength strikes their surface. This simple yet powerful principle helps solve cases that might otherwise remain mysteries.

Forensic teams harness these capabilities daily. For example, a bloodstain cleaned from a carpet fluoresces under forensic UV light. Document alterations emerge under infrared examination. Trace evidence hidden for years suddenly appears when struck by the correct wavelength. Each discovery adds another piece to complex investigative puzzles.

This article delves into the science of ALS light, its underlying technology, practical applications, and proper usage in modern crime scene investigation. From understanding basic principles to mastering advanced detection methods, discover how these tools illuminate the path to truth in criminal cases.

Understanding Alternative Light Source Technology

Picture a crime scene investigator examining a seemingly clean carpet. Under normal light, nothing appears unusual. The investigator switches on a crime light ranging from 300nm to 1200nm, and suddenly, crucial evidence emerges from the shadows.

Basic Principles of Light-Based Detection

Alternative light source forensics work through three fundamental mechanisms:

  • Fluorescence – Evidence absorbs light energy and glows at longer wavelengths
  • Absorption – Materials trap specific wavelengths, creating dark contrasts
  • Oblique lighting forensics – Angled illumination reveals tiny surface details

These interactions create a fascinating dance between light and evidence. Specialized filters block unwanted scattered light while capturing fluorescent emissions. The result? Fingerprints, bodily fluids, and trace evidence stand out clearly against complex backgrounds.

Types of Forensic Light Sources Available

The journey of forensic light technology tells an interesting story. The Royal Canadian Mounted Police first harnessed argon-ion lasers in the 1970s, marking a new era in evidence detection. Today’s investigators carry portable ALS kits that their predecessors could only dream about.

Key Components and Their Functions

Modern crime scene lights combine several critical elements:

  • Light Generation Units
    • Powerful lamps producing ultra-violet, visible, and infrared light
    • High-intensity LEDs maintain steady output despite battery drain
  • Filtering Systems
    • Specialized filters separating light into precise wavelength bands
    • Protective goggles ensure safe operation
  • Control Systems
    • WavelengthWavelength is a fundamental concept in the study of waves, whether they are associated with sound or light. It refers to the distance between two successive points on a wave, commonly measured from crest to crest or from trough to trough. Here are key points about wavelength: • Wave Nature: Sound waves and electromagnetic waves, including light, exhibit wave-like behavior. Wavelength is a characteristic feature of these waves.
      • Crest and Trough: A wave is characterized by its repeating pattern of crests (the highest points or peaks) and troughs (the lowest points or valleys). The distance between these successive crests or troughs is the wavelength.
      • Measurement: Wavelength is typically measured in units of length, such as meters (m) or centimeters (cm). It represents the spatial extent of one complete cycle of the wave.
      • Frequency Relationship: Wavelength and frequency are inversely related. Higher frequencies correspond to shorter wavelengths, and lower frequencies correspond to longer wavelengths. This relationship is described by the wave equation: speed = frequency × wavelength.
      • Sound Waves: In the context of sound waves, wavelength determines the pitch or tone of the sound. Shorter wavelengths correspond to higher-pitched sounds, while longer wavelengths correspond to lower-pitched sounds.
      • Light Waves: In the context of light waves, wavelength is associated with the color of light. Different colors of light have different wavelengths. For example, blue light typically has a shorter wavelength than red light.
      • Electromagnetic Spectrum: The electromagnetic spectrum encompasses a wide range of electromagnetic waves with varying wavelengths, including radio waves, microwaves, infrared, visible light, ultraviolet, X-rays, and gamma rays. Each portion of the spectrum corresponds to specific wavelength ranges.
      • Applications: Understanding wavelengths is fundamental in various scientific and technological applications, such as telecommunications, spectroscopy, and optics. It is essential for designing and working with wave-based technologies.
      • Wave Interference: Wavelength plays a critical role in phenomena like wave interference, where waves combine and interact with each other based on their wavelengths.
      • Propagation: Wavelength affects how waves propagate through different media. Some materials may affect certain wavelengths more than others, leading to phenomena like refraction and dispersion.
      • Frequency and Wavelength Relationship: The speed of a wave remains constant as it changes from one medium to another, so changes in wavelength are accompanied by corresponding changes in frequency to maintain this speed.
      In summary, wavelength is a fundamental property of waves, representing the distance between successive crests or troughs. It is a key factor in determining the characteristics of waves, including their color (in the case of light) and pitch (in the case of sound), and it plays a central role in various scientific and technological fields. More
      selectors for optimal evidence detection
    • Power management for consistent field performance

These components work together like a well-orchestrated symphony, revealing evidence on diverse surfaces and in challenging environmental conditions. From dusty crime scenes to pristine laboratories, ALS lights have become indispensable tools in pursuing justice.

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UV to IR Spectrum in Evidence Detection

The electromagnetic spectrum holds secrets invisible to human eyes. Each wavelength band tells its own story, from ultraviolet revelations to infrared discoveries. Discover how forensic evidence detection harnesses these invisible powers to uncover crucial clues.

UV Light Applications (300-400nm)

Ultraviolet light reveals nature’s hidden signatures. Biological materials betray their presence through distinctive fluorescence under UV illumination. The shallow penetration of UV rays makes them perfect detectives for surface evidence:

  • Biological fluid detection through natural fluorescence
  • Surface topology examination
  • Minute texture changes invisible to naked eyes

The 300-400nm range proves especially valuable for examining bone fragments, teeth, and certain drug residues. What other evidence might lurk beneath seemingly clean surfaces?

Visible Light Range Uses (400-700nm)

Blood tells tales under visible light’s watchful gaze. Scientific studies paint an impressive picture – wavelengths between 400-470nm increased visibility in 52% of previously hidden bruises. The visible spectrum’s detective work includes:

Violet Light (400-430nm)

  • Enhanced injury visibility
  • Consistent performance across skin types

Blue Light (430-470nm)

  • Superior bruise detection
  • Detailed pattern revelation

IR Detection Capabilities (700nm+)

Infrared radiation penetrates deeper than its shorter-wavelength cousins, revealing evidence that others miss. Operating above 700nm, IR light excels at examining questioned documents and deep tissue analysis. Picture a questioned document appearing normal under standard light—IR imaging suddenly reveals hidden alterations.

IR reflection creates striking contrasts between evidence and backgrounds identical to human eyes. From latent fingerprint detection to gunshot residue detection, IR light unveils crucial details that might otherwise escape notice.

Time matters in evidence detection. UV techniques work best within 2-3 days post-trauma, while IR imaging maintains its keen eye across various conditions and aging periods. Through careful wavelength selection, investigators maximize their chances of finding critical evidence while avoiding potential pitfalls.

Materials and Methods for Light Source Analysis

Alternative light source forensics demands meticulous attention to equipment setup and safety protocols. These fundamental preparations often determine the difference between success and failure.

Equipment Setup Requirements

The darkroom serves as command central for forensic light analysis. Ambient light diminishes evidence visibility, demanding careful control of environmental conditions. Equipment needs vary from portable ALS kits running on batteries to sophisticated systems requiring 110V power sources.

Bench-Mounted Systems

  • Proper ventilation for unrestricted cooling airflow
  • 20-minute operational intervals
  • 15-20 minute cooling periods between sessions
  • 15-meter minimum safe distance from personnel (49 feet)

Safety Protocols and Protective Gear

Safety protocols protect both evidence integrity and investigator well-being. Standard protective equipment includes:

Essential Protection

  • ANSI Z80.3 certified eyewear (100% UVA/UVB blocking)
  • Single-use evidence handling gloves
  • Protective clothing (lab coats, coveralls)
  • Facial shields and respirators
  • Contamination-preventing shoe covers

Light beam exposure demands strict precautions. Reflected beams can cause permanent eye damage. Orange protective goggles shield against filtered light but offer no protection from white light beyond 20 minutes. White light operations permit safe working distance allows total exposure times of 7 minutes for direct beam contact.

Additional Safety Measures

  • Protective clothing minimizes skin exposure risks
  • Enhanced ventilation for blood enhancement procedures
  • Modern passive cooling systems prevent cross-contamination

The laboratory becomes a fortress of safety through these protocols. Each measure, from proper ventilation to protective gear, builds upon the others to create a secure environment for evidence analysis..

Evidence Types and Detection Methods

The dance between light and evidence reveals hidden stories at crime scenes. Each type of evidence demands its own wavelength selection and examination approach, turning invisible clues into valuable forensic evidence.

Body Fluid Detection Techniques

Biological fluids emit unique fluorescent signatures that aid forensic investigations:

  • Semen: Glows brightest under forensic light, detectable even at a 1/100 dilution on white cotton.
  • Saliva & Vaginal Fluids: Fluoresce under specific wavelengths, making them identifiable on various surfaces.
  • Blood: Although blood absorbs light rather than fluoresces, it becomes visible using special light sources.

Forensic experts use longer wavelengths instead of traditional UV black lights to minimize background noise and enhance detection. This enables accurate identification of stains on mattresses, carpets, sheets, and clothing without excessive sampling.

Fingerprint Enhancement Procedures

Fingerprints reveal their secrets through forensic light examination and enhancement techniques:

Detection & Enhancement Steps

The examination unfolds through careful steps:

  • Quality Assessment: Examining clarity, pattern classification, and ridge details.
  • Verification Process: Independent examiner review, digital documentation, and quality assurance checks.

Studies indicate that light source analysis correlates with aluminum powder and chemical enhancement methods, with only a 4% overlap.

  • DFO (1,8-Diazafluoren-9-one) enhances latent prints when paired with forensic light sources.
  • Forensic Light Sources reveal fingerprintsFingerprint, impression made by the papillary ridges on the ends of the fingers and thumbs. Fingerprints afford an infallible means of personal identification, because the ridge arrangement on every finger of every human being is unique and does not alter with growth or age. Fingerprints serve to reveal an individual’s true identity despite personal denial, assumed names, or changes in personal appearance resulting from age, disease, plastic surgery, or accident. The practice of utilizing fingerprints as a means of identification, referred to as dactyloscopy, is an indispensable aid to modern law enforcement. More on unconventional surfaces such as plastic bags, duct tape, aluminum foil, wood, bricks, and glossy paper.
  • Multiple wavelengths allow detection on textured, contaminated, or background-masked surfaces.

Trace EvidenceTrace evidence - Physical evidence that results from the transfer of small quantities of materials (e.g., hair, textile fibers, paint chips, glass fragments, gunshot residue particles). More Visualization

Trace evidence examination unlocks connections between crime scenes, victims, and suspects. Light sources reveal distinctive patterns across various materials:

Physical Evidence

  • Paint fragments speaking of vehicle collisions
  • Fibers linking suspects to locations
  • Glass particles mapping crime scenes
  • Gunshot residue telling ballistic tales
  • Soil traces tracking movements

Multi-spectral imaging peers into the invisible spectrum, uncovering evidence that human eyes miss. Scanning electron microscopy adds another layer, revealing surface details and elemental compositions. Together, these tools weave fragments of evidence into compelling investigative narratives.

Hair and Fiber Detection

Hair and fiber analysis benefits significantly from using alternative light sources, revealing minute details and characteristics. Two main techniques are used:

  1. Oblique Lighting: Strong white light illuminates floors and carpets to reveal small particles.
  2. Fluorescent Lighting: UV or blue light causes certain hair and fibers to glow, making them stand out.

Combining both methods ensures comprehensive trace evidence collection.

Bruises, Bite Marks, and Pattern Wounds

Injury examination benefits from forensic light sources:

  • Different wavelengths penetrate varying skin depths, allowing bruise patterns and wound details to be analyzed.
  • Infrared illumination reveals deep wounds, while shorter wavelengths highlight surface injuries.

These techniques help investigators:

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  • Identify the weapon used based on bruise patterns.
  • Detect bite marks and shoe prints, linking suspects to victims.

Questioned Document Examination

Ink analysis exposes document forgeries:

  • Tunable Forensic Light Sources detect subtle differences in ink formulations.
  • Infrared (IR) analysis reveals erased text and altered writing.
  • Specialized IR-sensitive cameras visualize invisible details, helping forensic document examiners authenticate and analyze fraudulent documents.

Gunshot Residues and Explosive Detection

Certain ammunition and explosive residues fluoresce under forensic light sources:

  • Residues on shooters’ hands and clothing provide links between a suspect and a weapon.
  • Scene analysis identifies bullet trajectories and shooting positions.
  • Tunable light sources enhance non-invasive residue detection.

While effective, factors such as weather conditions, distance, and ammunition type influence results.

Detection of Human Bone Fragments, Tire Tracks, Shoe Prints, and Drugs

Forensic Light Sources aid in detecting:

  • Bone fragments: Differentiated from surrounding materials using spectral techniques.
  • Tire tracks & shoe prints: Enhanced using specific wavelength applications.
  • Drugs and chemical residues: Identified through fluorescence under selected forensic lights.

Forensic Light Sources have revolutionized crime scene investigation, allowing experts to accurately detect biological fluids, fingerprints, trace evidence, questioned documents, and gunshot residues. By utilizing multi-wavelength forensic tools, investigators can analyze complex surfaces, detect hidden evidence, and build stronger forensic cases.

Advanced Detection Techniques

The marriage of light and digital technology creates powerful new tools for evidence detection. Modern forensic laboratories showcase capabilities that early investigators could only imagine.

Multi-Wavelength Scanning Methods

Picture a forensic scanner that thinks for itself. Advanced systems now feature intelligent optics and automated filter selection for precise evidence detection. The Crime-lite ML PRO exemplifies this evolution—a semi-automated tool scanning the the UV-Vis-IR spectrum with pre-programmed modes. The system’s brain processes approximately 19,000 images of evidence samples, determining optimal detection settings across diverse materials.

Digital Documentation Systems

Modern imaging systems serve as both cameras and analysts. These sophisticated tools combine:

Visualization Technology

  • 10-inch touchscreens paired with 32-inch monitors
  • 29MP sensors capturing microscopic detail
  • UV reflectance imaging capabilities
  • Multi-spectral evidence capture

Evidence flows seamlessly through these systems while maintaining strict chain-of-custody records. Touchless analysis preserves sample integrity, a crucial advantage over traditional methods. Fluorescent evidence photography techniques have revolutionized how crime scene evidence is documented and analyzed.

Real-Time Analysis Tools

Real-time analysis capabilities transform crime scene processing from hours to minutes. Smart algorithms suggest optimal light and filter combinations based on evidence type and surface material. Evidence locations, bodily fluid examination, and trace evidence detection happen before an investigator’s eyes.

The latest systems include verification libraries, which ensure both proper use and result validation. These unified instruments bridge historical gaps between field investigation and laboratory analysis. Through careful integration, these tools contribute to gold-standard datasets that validate examination protocols through empirical testing.

What mysteries might tomorrow’s technology unlock? The fusion of light, digital imaging, and artificial intelligence continues pushing the boundaries of forensic science. Monolith forensics systems represent the cutting edge of this technological evolution, offering unprecedented capabilities in evidence detection and analysis.

System Limitations and Challenges

Despite their remarkable capabilities, alternative light sources face real-world constraints. Understanding these limitations helps investigators distinguish between scientific possibility and practical reality.

Environmental Interference Factors

The environment shapes detection success. Working distance proves critical—optimal ranges are between 2 and 10 meters indoors, with greater distances needed outdoors. Ambient light becomes either friend or foe, demanding carefully controlled examination conditions. Dark adaptation is crucial for investigators to maximize their visual sensitivity when working with ALS light.

Common Detection Obstacles

  • Skin conditions mimicking bruise patterns
  • Optical brighteners in cleaning products create false signals
  • Environmental contaminants triggering fluorescence
  • UV exposure threatening both DNA evidence and investigator safety

Material-Specific Detection Issues

Evidence detection becomes complex when different substrate materials come into play. Highly absorbent materials reduce detection capabilities, and blood detection suffers especially on materials like polar fleece. All the same, some evidence types remain detectable – semen stains can still be identified even on highly absorbent surfaces.

Surface color and composition add more challenges to the mix. Dark materials create special problems because they absorb all wavelengths of light, including both excitation and emission spectra. This means stains might look weaker or become impossible to detect on these surfaces. Materials with strong inherent fluorescence, like white cotton and pink satin, make it harder to see contrast between evidence and background.

Age-related factors add another layer of complexity. Detection effectiveness changes based on time between staining and examination. Time works against investigators:

  • Diluted blood samples are hard to detect on surfaces of all types, often producing false negatives whatever wavelength you choose.
  • Cleaning agents, especially bleach, interfere with both forensic light examination and later chemical testing.
  • Urine stains become harder to detect with time
  • Blood on dark clothing challenges detection through contrast issues and natural darkening

Each evidence type comes with its own set of limitations. The maximum detectable dilution for urine stains stays limited across different surfaces. Blood stains on light-colored surfaces are easy to spot, but finding them on dark clothing becomes much harder because of contrast issues and blood’s natural darkening over time.

Conclusion

ALS light illuminates paths to truth in modern criminal investigation. From ultraviolet revelations to infrared discoveries, these sophisticated tools reveal crucial evidence through spectral analysis that human eyes might never detect.

The story of forensic light detection unfolds across the electromagnetic spectrum. Forensic UV light exposes biological secrets, visible wavelengths map injury patterns, and infrared capabilities peer into questioned documents. Each wavelength band adds another chapter to complex criminal investigations.

Modern Capabilities

  • Multi-spectral examination unlocks hidden evidence
  • Digital documentation preserves crucial findings
  • Automated systems ensure consistent results.

Yet, every tool has its limits. Environmental factors challenge detection capabilities. Material properties sometimes shield evidence from discovery. Success demands understanding both the power and constraints of these remarkable instruments.

The future beckons with possibilities. Each technological advance brings new detection capabilities. Each solved case adds to our understanding. Forensic science continues its endless pursuit of truth and justice through careful application of these tools.

What mysteries will tomorrow’s light sources reveal? The answer lies in the ongoing dialogue between scientific innovation and criminal investigation.


FAQs

What are the main types of forensic light sources used in crime scene investigations?

Forensic light sources typically include high-intensity ultraviolet (UV), visible, and infrared (IR) light. These sources allow investigators to detect various types of evidence across the electromagnetic spectrum.

How does UV light assist in forensic analysis?

UV light is particularly effective in detecting biological materials and surface evidence. It can reveal bodily fluids, enhance fingerprints on difficult surfaces, and help examine wounds or skin trauma that may not be visible to the naked eye.

What role does infrared light play in forensic examinations?

Infrared light is valuable for document examination and deep tissue analysis. It can penetrate materials more deeply than other wavelengths, making it useful for detecting alterations in documents, analyzing evidence on dark surfaces, and enhancing fingerprint visibility.

What are some limitations of forensic light source technology?

Forensic light sources can be affected by environmental factors such as ambient lighting and working distance. Additionally, certain materials and surface colors can interfere with detection capabilities. The age of evidence and the presence of contaminants or cleaning agents can also impact the effectiveness of these tools.

Are there any safety concerns when using forensic light sources? 

Yes, there are safety concerns. Proper protective gear, including specialized eyewear, gloves, and clothing, is essential. Direct exposure to light beams should be avoided to prevent potential eye damage, and prolonged skin exposure should be minimized.

Further Readings & Sources

[1] – https://www.sciencedirect.com/science/article/abs/pii/S1355030622001575
[2] – https://www.west-technology.co.uk/forensic/forensic-light-sources-2/
[3] – https://www.crime-scene-investigator.net/alternatelightsources.html
[4] – https://simplyforensic.com/forensic-light-sources-in-forensic-science/
[5] – https://fosterfreeman.com/why-use-a-forensic-light-source/
[6] – https://www.ojp.gov/ncjrs/virtual-library/abstracts/ultraviolet-forensic-imaging
[7] – https://fosterfreeman.com/crime-lite-82l/
[8] – https://pmc.ncbi.nlm.nih.gov/articles/PMC11306313/
[9] – https://godoymedical.net/how-alternative-light-sources-are-used-in-forensics/
[10] – https://www.swgde.org/wp-content/uploads/2023/11/2020-09-17-SWGDE-Guideline-for-the-use-of-Infrared-Radiation-IR-in-Forensic-Photography_v1.0.pdf
[11] – https://fosterfreeman.com/crime-lite-applications-forensic-applications-of-infrared-light/
[12] – https://crimelab.phoenix.gov/QTDocuments/1700.PDF
[13] – https://www.sirchie.com/forensics/personal-protection.html
[14] – https://www.sbsheriff.org/wp-content/uploads/2019/12/SOP-CSI-002-12-Crime-Scene-Safety.pdf
[15] – https://regulaforensics.com/products/crime-scene-investigation/3116/
[16] – https://www.sciencedirect.com/science/article/pii/S2665910724000409
[17] – https://www.horiba.com/fileadmin/uploads/Scientific/Documents/Forensics/fls.pdf
[18] – https://www.researchgate.net/publication/228488113_Forensic_Light_Sources_for_Detection_of_Biological_Evidences_in_Crime_Scene_Investigation_A_Review
[19] – https://www.nj.gov/njsp/division/investigations/trace-evidence.shtml
[20] – https://www.nanoscience.com/blogs/what-role-does-sem-play-in-trace-evidence-analysis/
[21] – https://crimesciences.com/specialized-areas/forensic-light-sources-and-lasers/forensic-multi-wavelength-lights/
[22] – https://www.findlight.net/spectral-analysis/material-analysis-and-forensics/forensics/forensic-multi-spectral-fingerprints-photography-system-or-gdz10
[23] – https://fosterfreeman.com/crime-scene/
[24] – https://www.mdpi.com/1424-8220/23/18/7736
[25] – https://www.horiba.com/int/scientific/applications/others/pages/detection-of-body-fluids-with-an-alternate-light-source/
[26] – https://www.sciencedirect.com/science/article/pii/S2468170921000643

TAGGED:Art Authenticationbody fluid detectionbruise and wound analysiscounterfeit detectionexplosive residue detectionFLSForensic Light Sourcesgun shot residuesgunshot residue detectionhair and fiber examinationhuman bone fragmentslatent fingerprint detectionphysical injuriesquestioned document examinationquestioned documentsshoe printstire tracksultra-violet
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Forensic Analyst by Profession. With Simplyforensic.com striving to provide a one-stop-all-in-one platform with accessible, reliable, and media-rich content related to forensic science. Education background in B.Sc.Biotechnology and Master of Science in forensic science.
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